HEADER MEMBRANE PROTEIN 02-OCT-03 1R3I . COMPND 2 MOLECULE: ANTIBODY FAB FRAGMENT LIGHT CHAIN; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR Y.ZHOU,R.MACKINNON . flagSetFrame DONE ==== Generated by WOLFc v1.01 (last modified 28.04.2002) . ==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=2-APR-2009 . ==== REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . 534 # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA X-CB Y-CB Z-CB N-H.O=C C=O.H-N N-H.O=C C=O.H-N N-H.O=C C=O.H-N X1 Y1 Z1 X2 Y2 Z2 X3 Y3 Z3 1 1 L D 0 0 111 0, 0.0 2,-0.2 0, 0.0 95,-0.1 0.000 360.0 360.0 360.0 125.4 154.6 140.1 -12.6 154.8 138.7 -11.9 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.9057 0.1005 0.4119 -0.3292 0.4455 -0.8326 -0.2672 -0.8896 -0.3704 2 2 L I - 0 0 7 93,-0.3 2,-0.5 91,-0.2 88,-0.1 0.000 360.0-149.0 -68.3 126.4 151.5 142.2 -11.7 150.4 142.6 -12.7 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.6347 0.1342 0.7610 0.7432 0.3757 0.5536 -0.2116 0.9170 -0.3382 3 3 L L - 0 0 109 -2,-0.2 23,-2.3 94,-0.1 2,-0.6 0.000 4.9-157.0 -97.7 130.9 150.4 142.1 -8.1 151.3 142.5 -6.9 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.7944 0.1336 0.5926 -0.4498 -0.7849 -0.4261 0.4082 -0.6051 0.6836 4 4 L L E -A 25 0A 6 -2,-0.5 96,-0.7 94,-0.4 2,-0.6 0.000 7.3-159.6-111.3 113.3 146.7 142.5 -7.3 145.7 141.6 -8.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.6925 0.1890 0.6962 0.3690 0.9221 0.1167 -0.6199 0.3377 -0.7083 5 5 L T E -A 24 0A 68 19,-3.5 19,-2.7 -2,-0.6 2,-0.6 0.000 7.7-173.1 -98.6 121.4 145.9 143.7 -3.8 146.7 144.9 -3.2 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.7218 0.1247 0.6808 -0.1491 -0.9886 0.0231 0.6759 -0.0848 0.7321 6 6 L Q - 0 0 5 -2,-0.6 17,-0.2 17,-0.2 -2,-0.0 0.000 20.3-158.7-114.7 103.5 142.4 143.1 -2.4 141.6 141.8 -2.8 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.6247 0.1097 0.7731 0.0387 0.9932 -0.1096 -0.7799 -0.0385 -0.6247 7 7 L S + 0 0 79 -2,-0.6 2,-0.1 15,-0.2 15,-0.1 0.000 65.3 37.6-140.1 139.3 141.9 144.8 0.9 143.2 145.4 1.4 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.5199 0.5244 0.6743 -0.6243 -0.7720 0.1190 0.5830 -0.3591 0.7288 8 8 L P S S- 0 0 66 0, 0.0 14,-0.2 0, 0.0 3,-0.1 0.000 71.3-152.3 -69.0 160.0 140.1 144.5 3.2 139.1 144.9 4.3 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.0780 -0.9776 0.1957 0.9647 -0.1236 -0.2326 0.2516 0.1706 0.9527 9 9 L A S S+ 0 0 62 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H F B +Z 335 0I 9 -29,-1.9 -29,-3.2 -2,-0.3 2,-0.1 0.000 61.6 17.7-171.6 138.4 138.3 108.6 2.2 139.4 108.1 3.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.6524 0.1029 -0.7508 -0.4650 0.8367 -0.2894 0.5984 0.5379 0.5937 364 152 H P S S- 0 0 0 0, 0.0 -1,-0.2 0, 0.0 54,-0.2 0.000 92.9 -76.1 -88.2-170.8 139.5 110.7 0.4 140.5 111.0 -0.6 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.3805 0.9226 0.0633 -0.2422 -0.1655 0.9560 0.8925 0.3484 0.2864 365 153 H E S S+ 0 0 48 -2,-0.1 2,-0.1 -3,-0.1 -38,-0.1 0.000 78.0 51.6 -80.8 161.8 137.3 113.8 0.0 137.6 115.1 0.6 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.5099 0.4571 -0.7287 -0.6398 -0.7678 -0.0340 -0.5751 0.4489 0.6840 366 154 H P + 0 0 33 0, 0.0 52,-0.4 0, 0.0 2,-0.3 0.000 37.6 176.0-109.1-171.8 134.8 114.8 -1.2 134.5 116.1 -1.9 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.9468 -0.2420 0.2124 0.3092 -0.4995 0.8093 -0.0897 0.8318 0.5477 367 155 H V - 0 0 16 49,-0.1 2,-0.5 50,-0.1 49,-0.2 0.000 21.7-142.3-134.6 149.7 131.3 113.4 -1.2 130.8 112.7 0.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.9597 0.1490 -0.2381 0.2254 -0.0973 -0.9694 -0.1676 -0.9840 0.0598 368 156 H T E -E 416 0K 82 47,-2.2 47,-2.5 -2,-0.3 2,-0.5 0.000 15.4-161.7-119.5 119.1 128.3 115.2 -2.7 128.2 115.4 -4.2 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.9848 0.1685 0.0429 0.1166 0.4571 0.8818 0.1290 0.8733 -0.4697 369 157 H V E +E 415 0K 18 -2,-0.5 2,-0.3 45,-0.2 45,-0.2 0.000 13.4 171.5-106.7 127.7 125.0 114.9 -0.8 125.0 115.0 0.8 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.9910 0.1336 0.0000 -0.0885 -0.6562 -0.7494 -0.1001 -0.7427 0.6621 370 158 H T E -E 414 0K 41 43,-2.6 43,-3.3 -2,-0.5 2,-0.5 0.000 26.2-129.3-126.6 164.2 121.6 115.6 -2.4 121.5 115.4 -3.9 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.9979 0.0000 0.0645 0.0470 0.6855 0.7265 -0.0442 0.7281 -0.6841 371 159 H W E >S-EF 413 377K 2 5,-0.5 5,-2.1 -2,-0.3 3,-0.5 0.000 85.3 -9.1-120.8 127.7 118.0 115.1 -1.2 117.5 116.0 -0.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.9340 -0.2843 -0.2163 0.3235 -0.9300 -0.1745 -0.1516 -0.2329 0.9606 372 160 H N T > 5S- 0 0 37 39,-3.6 3,-2.0 -2,-0.5 -1,-0.2 0.000 131.1 -53.7 51.7 52.1 115.5 113.3 -3.4 114.1 114.0 -3.2 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.1814 -0.0508 -0.9821 0.4402 -0.8972 -0.0349 -0.8794 -0.4386 0.1851 373 161 H S T 3 5S- 0 0 102 38,-0.3 -1,-0.2 1,-0.3 39,-0.1 0.000 106.3 -53.0 57.2 32.0 117.9 113.2 -6.3 117.1 112.5 -7.3 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.3347 0.6457 -0.6863 0.8794 0.0476 0.4737 0.3385 -0.7621 -0.5519 374 162 H G T 3 5S+ 0 0 44 -3,-0.5 -1,-0.3 2,-0.2 -2,-0.2 0.000 114.6 111.6 91.2 -12.4 118.4 117.0 -6.1 119.1 117.0 -7.4 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.4317 0.8888 -0.1537 0.0916 0.2127 0.9728 0.8974 0.4059 -0.1733 375 163 H S T < 5S+ 0 0 97 -3,-2.0 2,-0.6 1,-0.2 -3,-0.2 0.000 73.1 60.6 -69.0 -13.9 114.7 117.9 -6.2 113.5 116.9 -6.5 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.6367 0.5957 0.4897 0.4729 0.8032 -0.3623 -0.6091 0.0009 -0.7931 376 164 H L B < +F 372 0K 44 -5,-2.1 -5,-0.5 1,-0.1 -2,-0.2 0.000 56.6 140.4-119.5 101.7 114.9 119.0 -2.6 114.5 118.0 -1.5 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.4458 0.5817 0.6804 -0.3197 0.8134 -0.4859 -0.8361 -0.0009 0.5486 377 165 H S > + 0 0 69 -2,-0.6 3,-0.9 -7,-0.1 2,-0.2 0.000 44.5 87.4-110.6 -32.1 117.4 121.9 -2.1 117.8 122.7 -3.4 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.4149 0.7058 0.5742 0.3258 -0.7045 0.6305 0.8495 -0.0745 -0.5222 378 166 H S T 3 S+ 0 0 77 1,-0.2 21,-0.3 20,-0.0 0, 0.0 0.000 92.1 22.2 -76.6 137.4 115.8 124.3 0.3 114.5 125.0 0.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 -0.1229 0.0939 0.9880 0.9871 0.1149 0.1119 -0.1031 0.9889 -0.1068 379 167 H G T 3 S+ 0 0 37 1,-0.3 20,-0.8 -2,-0.2 2,-0.4 0.000 90.9 135.7 83.2 20.1 116.4 123.7 4.0 115.0 123.1 4.3 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.4399 -0.6420 0.6279 0.6394 0.7149 0.2829 -0.6305 0.2770 0.7250 380 168 H V E < +C 399 0J 33 -3,-0.9 2,-0.4 18,-0.2 -1,-0.3 0.000 29.3 178.7-108.4 133.6 119.6 121.6 3.5 119.8 121.0 2.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.9617 -0.1483 0.2307 -0.2549 -0.1725 0.9515 -0.1013 -0.9738 -0.2037 381 169 H H E -C 398 0J 12 16,-2.0 16,-3.3 -2,-0.4 2,-0.7 0.000 5.6-172.2-130.1 115.9 122.9 122.0 5.5 122.8 122.4 7.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.9200 -0.3348 0.2037 0.2999 0.2669 -0.9159 0.2523 0.9037 0.3460 382 170 H T E -C 397 0J 55 -2,-0.4 14,-0.2 14,-0.3 3,-0.1 0.000 22.4-143.1-111.2 108.1 125.8 119.7 4.6 126.1 119.2 3.2 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.9335 -0.2385 0.2679 -0.3297 -0.2766 0.9026 -0.1412 -0.9309 -0.3369 383 171 H F - 0 0 1 12,-2.0-220,-0.1 -2,-0.7 12,-0.1 0.000 24.1 -96.1 -69.7 149.6 128.4 120.2 7.3 128.3 119.8 8.7 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.9962 -0.0101 0.0862 0.0585 0.8120 -0.5808 -0.0641 0.5836 0.8095 384 172 H P - 0 0 59 0, 0.0 11,-0.2 0, 0.0 -1,-0.1 0.000 48.8-104.0 -62.3 148.6 132.2 120.2 6.4 133.0 121.4 6.8 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.7283 -0.6780 0.0995 -0.3807 -0.5211 -0.7638 0.5697 0.5184 -0.6377 385 173 H A - 0 0 22 9,-0.1 2,-0.4 -3,-0.1 9,-0.2 0.000 24.9-143.3 -74.9 158.7 133.8 116.8 7.0 134.4 115.9 6.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 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C Y - 0 0 132 -2,-0.3 -24,-0.4 -3,-0.1 -23,-0.3 0.000 45.6 -93.8-168.8 149.1 154.8 145.8 -29.4 155.9 146.9 -29.4 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.6917 -0.7122 -0.1195 -0.6346 -0.5204 -0.5714 0.3448 0.4710 -0.8119 493 83 C P - 0 0 12 0, 0.0 -36,-0.0 0, 0.0 -2,-0.0 0.000 23.9-175.0 -70.2 147.1 156.5 142.4 -29.3 156.7 141.6 -30.6 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.7428 -0.4527 0.4933 -0.5424 0.0251 0.8397 -0.3925 -0.8913 -0.2269 494 84 C V + 0 0 58 -2,-0.1 2,-0.2 -30,-0.1 3,-0.0 0.000 61.4 77.4-116.4 -8.9 159.2 141.6 -26.7 158.9 141.8 -25.2 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.8216 -0.5221 0.2290 0.4835 0.4254 -0.7650 0.3020 0.7393 0.6019 495 85 C T S > S- 0 0 27 1,-0.1 4,-2.5 -31,-0.1 5,-0.2 0.000 84.1-116.0-103.3 161.6 160.2 138.1 -28.0 159.3 136.8 -27.8 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0.5114 -0.5255 -0.6800 0.8473 0.4406 0.2967 0.1437 -0.7279 0.6705 496 86 C L H > S+ 0 0 123 -2,-0.2 4,-2.2 1,-0.2 5,-0.2 0.000 116.4 43.7 -56.4 -54.2 162.3 137.0 -30.9 163.4 135.9 -30.9 0 0.0 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